Plasmid Preparation:Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).
Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).
Cloning:Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).
Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).
Polymerase Chain Reaction:Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).
Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).
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